The brain samples were collected at 24 hours after SAH. Western blotting was performed as described previously18 (link). Primary antibodies used were: MFGE8 (Santa Cruz Biotechnology), HO-1, Nrf2 (Santa Cruz Biotechnology), 4-Hydroxynonenal (4-HNE, Abcam Biotech Company), Nitrotyrosine (Santa Cruz Biotechnology), integrin β3, extracellular-signal-regulated kinase (ERK) and phosphorylated ERK (p-ERK).
Protocol detail
Protein Expression Analysis of Brain Samples after SAH
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4 hydroxynonenal
Antibodies
Brain
Extracellular signal regulated kinase
Integrin
Mfge8
Nitrotyrosine
Nrf2
Corresponding Organization :
Other organizations : Third Xiangya Hospital, Loma Linda University, Central South University
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Variable analysis
independent variables
- Time after SAH (24 hours)
- MFGE8 expression
- HO-1 expression
- Nrf2 expression
- 4-Hydroxynonenal (4-HNE) expression
- Nitrotyrosine expression
- Integrin β3 expression
- Extracellular-signal-regulated kinase (ERK) expression
- Phosphorylated ERK (p-ERK) expression
- Western blotting protocol as described previously in reference 18
- No positive or negative controls were explicitly mentioned in the provided information.
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