RNA was extracted from algal cells on the 24th hour of tunicamycin stress using the RNAprep pure Plant Kit [Tiangen Biotech (Beijing) Co., Ltd., Beijing, China] and measured using NanoDrop 2000 (Thermo). RNA integrity was analyzed using the RNANano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, United States). cDNA was synthesized using random primers [cDNA synthesis kit, Tiangen Biotech (Beijing) Co., Ltd.]. NEB Next Ultra TMRNA Library Prep Kit for Illumina (NEB, United States) was used to generate sequence libraries. Sequence reads are available on the NCBI sequence read archives [GSE209809].
The library construction of transcriptomic and bioinformatic analysis were conducted as described in our previous paper [31 (link)]. Genes with |fold change|≥ 1.5 and FDR < 0.01 (adjusted P-value, determined by the Benjamini and Hochberg multiple-testing correction implemented in the ‘p. adjust’ method of R) were defined as differentially expressed genes. The value of FPKM was the average of three biological replicates.
The library construction of transcriptomic and bioinformatic analysis were conducted as described in our previous paper [31 (link)]. Genes with |fold change|≥ 1.5 and FDR < 0.01 (adjusted P-value, determined by the Benjamini and Hochberg multiple-testing correction implemented in the ‘p. adjust’ method of R) were defined as differentially expressed genes. The value of FPKM was the average of three biological replicates.
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