Multiparameter Immunophenotyping of Immune Cells

PerCp-Cy5.5 anti-CD161, PE anti-RORγt, PE anti–γδ TCR, biotinylated anti–γδ TCR, PerCp-Cy5.5 anti-CD45RA, and APC anti–αβ TCR antibodies were from eBioscience. A647 anti-PLZF, APC-Cy7 anti-CD4, PE-Cy7 anti-CD8, BV421 anti-CD3, BV421 anti-CD161, PE anti-STAT4 (pY693), PE anti–IL-6R, PE anti–IL-12Rβ1, PE anti–IL-21R, PE mouse IgG1 isotype control, PerCP-Cy5.5 anti-STAT3 (pY705), and FITC anti–Vδ2 TCR antibodies were from BD. FITC anti–Vα24 TCR and PE and biotinylated anti-Vβ11 antibodies were from Beckman Coulter. FITC anti–Vδ1 TCR was from Thermo Fisher Scientific. APC or APC-Cy7 anti-Vα7.2 and PE-Cy7 anti–αβ TCR antibodies were from BioLegend. FITC anti-CCR7 antibody was from R&D Systems. MR1–5-OP-RU tetramers have been described previously (Reantragoon et al., 2013 (link); Corbett et al., 2014 (link)).

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Wilson R.P., Ives M.L., Rao G., Lau A., Payne K., Kobayashi M., Arkwright P.D., Peake J., Wong M., Adelstein S., Smart J.M., French M.A., Fulcher D.A., Picard C., Bustamante J., Boisson-Dupuis S., Gray P., Stepensky P., Warnatz K., Freeman A.F., Rossjohn J., McCluskey J., Holland S.M., Casanova J.L., Uzel G., Ma C.S., Tangye S.G, & Deenick E.K. (2015). STAT3 is a critical cell-intrinsic regulator of human unconventional T cell numbers and function. The Journal of Experimental Medicine, 212(6), 855-864.

Publication 2015

PE anti-RORγt APC-Cy7 anti-CD4 PE-Cy7 anti-CD8 BV421 anti-CD3 BV421 anti-CD161 PE anti–IL-21R PE mouse IgG1 isotype control

Anti antibodies Anti antibody Anti cd3 Cd161 Cy5 5 Fitc Igg1 Il 21r Il 6r Isotype Mouse Rorγt Stat3 Stat4 Tetramers

Corresponding Organization : UNSW Sydney

Other organizations : University of Bath, Hiroshima University, University of Manchester, Royal Manchester Children's Hospital, Royal Children's Hospital, Children's Hospital at Westmead, Royal Prince Alfred Hospital, Royal Perth Hospital, University of Western Australia, Westmead Hospital, Inserm, Assistance Publique – Hôpitaux de Paris, Université Paris Cité, Institut des Maladies Génétiques Imagine, Rockefeller University, Hadassah Medical Center, University Medical Center Freiburg, University of Freiburg, National Institute of Allergy and Infectious Diseases, Australian Research Council, ARC Centre of Excellence in Advanced Molecular Imaging, Monash University, Cardiff University, Peter Doherty Institute, University of Melbourne, Howard Hughes Medical Institute

Top 5 similar protocols

Variable analysis

independent variables

PerCp-Cy5.5 anti-CD161
PE anti-RORγt
PE anti–γδ TCR
Biotinylated anti–γδ TCR
PerCp-Cy5.5 anti-CD45RA
APC anti–αβ TCR
A647 anti-PLZF
APC-Cy7 anti-CD4
PE-Cy7 anti-CD8
BV421 anti-CD3
BV421 anti-CD161
PE anti-STAT4 (pY693)
PE anti–IL-6R
PE anti–IL-12Rβ1
PE anti–IL-21R
PE mouse IgG1 isotype control
PerCP-Cy5.5 anti-STAT3 (pY705)
FITC anti–Vδ2 TCR
FITC anti–Vα24 TCR
PE and biotinylated anti-Vβ11
FITC anti–Vδ1 TCR
APC or APC-Cy7 anti-Vα7.2
PE-Cy7 anti–αβ TCR
FITC anti-CCR7
MR1–5-OP-RU tetramers

dependent variables

Not explicitly mentioned

control variables

PE mouse IgG1 isotype control

positive controls

MR1–5-OP-RU tetramers

negative controls

PE mouse IgG1 isotype control

Annotations

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