Mouse tissues were fixed in 10% (v/v) buffered formalin and embedded in paraffin. Sections of the paraffin-embedded tissues were subjected to immunohistochemical (IHC) analysis as described[8 (link)]. The following primary antibodies were used for IHC analysis: mouse anti–β-catenin (1:800; BD Biosciences, Lexington, KY), rabbit anti-p53 (1:500; Vector Laboratories Inc., Burlingame, CA), goat anti–E-cadherin (1:500; R&D Systems, Minneapolis, MN), Rabbit anti-vimentin (1:500; Epitomics, Burlingame, CA), and mouse anti-CDX2 (1:100; BioGenex, Fremont, CA). The IHC staining with anti-p53 antibody was classified according to the percentage of stained cells. Expression of p53 was considered to be weak if <10% of neoplastic cells were observed to be stained, and strong if >10% of neoplastic cells were stained.
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Immunohistochemical Analysis of Mouse Tissue
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Corresponding Organization : University of Michigan–Ann Arbor
Other organizations : Xiangya Hospital Central South University, Central South University, Hiroshima University
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- Primary antibodies used for IHC analysis: mouse anti–β-catenin, rabbit anti-p53, goat anti–E-cadherin, Rabbit anti-vimentin, and mouse anti-CDX2
- Expression of p53 (classified according to the percentage of stained cells)
- Mouse tissues were fixed in 10% (v/v) buffered formalin and embedded in paraffin
- Sections of the paraffin-embedded tissues were subjected to immunohistochemical (IHC) analysis as described[8]
- Not explicitly mentioned
- Not explicitly mentioned
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