Immunofluorescence Analysis of Meiotic Proteins

The following antibodies were used: rabbit antibodies against TERB1 (Shibuya et al., 2014 (link)), TERB2 (Shibuya et al., 2015 (link)), MAJIN (Shibuya et al., 2015), SYCE3 (Zhang et al., 2019 (link)), TRF2 (Novus Biologicals; NB110–57130), γH2AX (Abcam; ab11174), and SMC3 (Abcam; ab9263); mouse antibodies against TRF1 (Shibuya et al., 2014 (link)), β-actin (Sigma; A2228–100UL), and MLH1 (BD Biosciences; 51–1327GR); rat antibody against RPA2 (Cell Signaling Technology; 2208); guinea pig antiserum against histone H1T (Inselman et al., 2003 (link)); goat antibody against Lamin B (Santa Cruz Biotechnology; sc-6216); and chicken antibody against SYCP3 (Zhang et al., 2019 (link)).

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Zhang K., Tarczykowska A., Gupta D.K., Pendlebury D.F., Zuckerman C., Nandakumar J, & Shibuya H. (2022). The TERB1 MYB domain suppresses telomere erosion in meiotic prophase I. Cell reports, 38(4), 110289.

Publication 2022

NB110–57130 ab11174 ab9263 A2228–100UL sc-6216

Actin Antibodies Antibody Antibody b Antiserum Biologicals Chicken Goat Guinea pig Histone Lamin Lamin b Mlh1 Mouse Novus Rabbit Rpa2 Smc3

Corresponding Organization : University of Gothenburg

Other organizations : University of Michigan–Ann Arbor

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Variable analysis

independent variables

Rabbit antibodies against TERB1, TERB2, MAJIN, SYCE3, TRF2, γH2AX, and SMC3
Mouse antibodies against TRF1, β-actin, and MLH1
Rat antibody against RPA2
Guinea pig antiserum against histone H1T
Goat antibody against Lamin B
Chicken antibody against SYCP3

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: None specified
Negative controls: None specified

Annotations

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