Similarly, the β-galactosidase activity in the supernatant was tested via its corresponding kit (Solarbio, Beijing). β-galactosidase decomposes p-nitrobenzene-β-
Antibiotics Induced Membrane Damage in E. coli and K. pneumoniae
Similarly, the β-galactosidase activity in the supernatant was tested via its corresponding kit (Solarbio, Beijing). β-galactosidase decomposes p-nitrobenzene-β-
Corresponding Organization : Zhejiang Lab
Protocol cited in 3 other protocols
Variable analysis
- Quercetin (½ MIC)
- Colistin (½ MIC)
- Quercetin (½ MIC) + Colistin (½ MIC)
- Alkaline phosphatase (ALP) activity
- β-galactosidase activity
- Incubation temperature (37°C)
- Incubation time (24 h)
- Shaker speed (180 rpm)
- Bacterial strains (E. coli and K. pneumoniae)
- Initial bacterial cell density (OD600 = 0.5)
- Culture medium (LB broth)
- No positive or negative controls were explicitly mentioned in the input.
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
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