Microbial Cultivation and Biomass Determination

Bacillus subtilis CICC10012 (B. subtilis), Salmonella enterica WX29 (S. enterica), Staphyloccocus aureus CICC10201 (S. aureus), Bacillus cereus ATCC11778 (B. cereus), Escherichia coli CICC10899 (E. coli), Pseudomonas brenneri CICC10271 (P. brenneri) were purchased from the China Center of Industrial Culture Collection (Beijing, China) and were cultivated in Luria Bertani (LB) broth with aeration at 37 °C. L. rhamnosus YT, preserved by the key laboratory of dairy biotechnology and safety control of Jiangsu province, was isolated from the feces of Bama longevity, Guangxi province, China. L. rhamnosus YT was inoculated in dMRS broth at 37 °C in static condition. The biomass was determined by viable counts [39 (link)].

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Guan C., Zhang W., Su J., Li F., Chen D., Chen X., Huang Y., Gu R, & Zhang C. (2023). Antibacterial and antibiofilm potential of Lacticaseibacillus rhamnosus YT and its cell-surface extract. BMC Microbiology, 23, 12.

Publication 2023

Bacillus subtilis CICC10012 Salmonella enterica WX29

Bacillus cereus Bacillus subtilis E coli Feces Pseudomonas brenneri S aureus Safety Salmonella enterica

Corresponding Organization : Yangzhou University

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Variable analysis

independent variables

Bacillus subtilis CICC10012 (B. subtilis)
Salmonella enterica WX29 (S. enterica)
Staphyloccocus aureus CICC10201 (S. aureus)
Bacillus cereus ATCC11778 (B. cereus)
Escherichia coli CICC10899 (E. coli)
Pseudomonas brenneri CICC10271 (P. brenneri)
L. rhamnosus YT

dependent variables

Biomass determined by viable counts

control variables

Cultivation in Luria Bertani (LB) broth with aeration at 37 °C
Inoculation of L. rhamnosus YT in dMRS broth at 37 °C in static condition

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