Unless otherwise specified, all reagents and chemicals were purchased from Sigma-Aldrich Co. (St. Louis, MO). Anti-human CD3 antibody (OKT3) was kindly provide by Dr. Gilliland of Janssen and used for stimulation of all primary PBMC. Fluorescently labeled antibodies for flow cytometry against: CD8, CD4, CD3, CD62L, annexin V, and their isotype controls (IgG1, IgG2A, IgG2B) were obtained from BD Biosciences (San Jose, CA), BioLegend (San Diego, CA) or eBioscience (San Diego, CA). APC-labeled CD62L antibodies and BV421-labeled annexin V antibodies used for confocal microscopy were obtained from BioLegend (San Diego, CA) and BD Biosciences (San Jose, CA), respectively. HIV-1 core antigen antibody (KC57-FITC/PE) for intracellular p24 staining was purchased from Beckman Coulter, Inc. (Miami, FL). Recombinant IL-2, also known as Teceleukin or Tecin (RO23-6019, Roche, MN) was obtained from National Cancer Institute, NIH. The Luciferase Assay System was purchased from Promega Corporation (Madison, WI). HIV-1 p24 ELISA kit was obtained from PerkinElmer Life Sciences, Inc. (Waltham, MA). Ficoll-Paque was purchased from GE Healthcare Life Sciences (Pittsburgh, PA). The Vybrant FAM poly-caspase assay from Molecular Probes-Thermofisher (Waltham, MA) was used for intracellular, active-caspase staining of pan-caspases. For individual caspase expression measurements, caspase-1, -2, -3/7, -6, -8, -9, and -10 fluorescent inhibitors of caspases (FLICA) were used to stain active-caspases in live cells from Bio-Rad (Hercules, CA). DMEM, RPMI1640, penicillin/streptomycin (Pen/Strep), fetal bovine serum (FBS), and HEPES were purchased from Invitrogen Corporation (Carlsbad, CA). The metalloproteinase inhibitor, BB-94 (Batimastat), TAPI-1 (TNFα protease inhibitor-1) and TAPI-2 were purchased from Santa Cruz Biotechnology. Caspase inhibitors Z-VDVAD-FMK (caspase 2), and Z-DEVD-FMK (caspases 3,6,7, and 10) were purchased from Santa Cruz Biotechnology, Z-DQMD-FMK (caspase 3), Z-IETD-FMK (caspase 8) and Z-LEHD-FMK (caspase 9) were purchased from Adooq, Ac-DEVD-CHO (caspase 3, 7) and Ac-LEVD-CHO (caspase 4) were purchased from Sigma-Aldrich, Belnacasan (caspase 1 and 4) and pan-caspase inhibitors Z-VAD-FMK and Q-VD-OPH were purchased from MedKoo Biosicences (Morrisville, NC) or SM Biochemicals LLC (Anaheim, CA).
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