Zebrafish Nephron Regeneration Modulation

During the nephron regeneration stage, the Indo (I7378-5G, Sigma; 400 μM, 10 μL per fish), NS-398 (N194-5MG, Sigma; 140 μM, 10 μL per fish), TG4-155 (S6793, Selleck; 400 μM, 10 μL per fish), dmPGE2 (D0160, Sigma; 600 μM, 10 μL per fish), GW627368X (T1978, TOPSCIENCE; 200 μM, 10 μL per fish), XAV939 (S1180, Selleck; 200 μM, 10 μL per fish), ICRT 14 (HY-16665, MCE; 10 μM, 10 μL per fish), ICG-001 (HY-14428, MCE; 100 μM, 10 μL per fish), PKI (HY-P1290A, MCE; 20 μM, 10 μL per fish), and H89 (HY-15979, MCE; 200 μM, 10 μL per fish) were intraperitoneally injected into zebrafish at 2, 4, and 6 dpi. Kidneys were collected at 7 dpi for RNA extraction, WISH, or immunofluorescence. For the 5 dpi test, inhibitors were injected at 2 and 4 dpi, and samples were collected at 5 dpi; 1% DMSO was injected in control groups with the same conditions. For juvenile zebrafish experiments, dmPGE2 (2 μM in egg water) was used to test the influence on mesonephros development. 0.2% DMSO was used in control groups under the same conditions.