Phage DNA Extraction and Genome Sequencing

Phage DNA was extracted from the purified high-titer phage suspension using the phenol–chloroform method described previously [6 (link),33 (link)]. The genomic DNA was sequenced using the Illumina HiSeq 4000 platform by Shanghai Majorbio Bio-pharm Technology Co., Ltd. After quality control and trimming, a total of 2,192,371,253 bp clean reads were obtained. Afterward, the clean reads were assembled using IDBA-UD version 1.1.1 to generate the final complete genome sequence [35 (link)].

Free full text: Click here

Zheng H., Liu Y., Zhou R., Liu J., Xu Y, & Chen F. (2023). An Estuarine Cyanophage S-CREM1 Encodes Three Distinct Antitoxin Genes and a Large Number of Non-Coding RNA Genes. Viruses, 15(2), 380.

Publication 2023

Illumina HiSeq 4000

A 192 Chloroform Genome Phage Phenol

Corresponding Organization : University of Maryland Center for Environmental Science

Top 5 similar protocols

Variable analysis

independent variables

Phage DNA extraction method (phenol-chloroform)

dependent variables

Genomic DNA sequencing
Quality control and trimming of reads
Genome assembly using IDBA-UD

control variables

Not explicitly mentioned

controls

No positive or negative controls are explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!