Binding of human anti-HIV-1 Env and control monoclonal IgG antibodies (mGO53 [Wardemann et al., 2003 (link)] and ED38 [Meffre et al., 2004 (link)]) to HEp-2 cell–expressing autoantigens was analyzed at 100 µg/ml by indirect IFA (ANA HEp-2 AeskuSlides; Aesku.Diagnostics) following the manufacturer’s instructions in two independent experiments. IFA sections were examined using the fluorescence microscope Axio Imager 2 (Zeiss), and pictures were taken at magnification 40× with 5,000 ms–acquisition using ZEN imaging software (Zen 2.0 blue version; Zeiss) at the Photonic BioImaging platform (Institut Pasteur).
Protocol detail
Binding of Anti-HIV-1 Env Antibodies to HEp-2 Cells
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Inserm
Other organizations : Sorbonne Paris Cité, Université Paris Cité, Department of Virology, Université de Tours, Central European Institute of Technology, Central European Institute of Technology – Masaryk University, University of Amsterdam, Cornell University, Amsterdam University Medical Centers, Ragon Institute of MGH, MIT and Harvard, Harvard University, Massachusetts General Hospital, Rockefeller University, Centre de Recherche des Cordeliers, Sorbonne Université, Beth Israel Deaconess Medical Center, Institut de Chimie des Substances Naturelles, Université Paris-Saclay
Top 5 similar protocols
Variable analysis
independent variables
- Binding of human anti-HIV-1 Env and control monoclonal IgG antibodies (mGO53 and ED38) to HEp-2 cell–expressing autoantigens
- Binding of human anti-HIV-1 Env and control monoclonal IgG antibodies (mGO53 and ED38) to HEp-2 cell–expressing autoantigens analyzed at 100 µg/ml by indirect IFA
- Indirect IFA (ANA HEp-2 AeskuSlides; Aesku.Diagnostics) following the manufacturer's instructions
- Fluorescence microscope Axio Imager 2 (Zeiss) used to examine IFA sections
- Pictures taken at magnification 40× with 5,000 ms–acquisition using ZEN imaging software (Zen 2.0 blue version; Zeiss) at the Photonic BioImaging platform (Institut Pasteur)
- Positive control: mGO53 monoclonal IgG antibody
- Negative control: ED38 monoclonal IgG antibody
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!