Muramyl dipeptide (MDP) and mouse recombinant IL-1β were purchased commercially (Bachem, Torrance, CA and R&D Systems, Minneapolis, MN, respectively). IL-1Ra, IL-1α, and IL-1β protein levels were measured in whole eye homogenates using commercially available ELISA kits (R&D Systems) as per manufacturer’s instructions and previously reported (16 (link)).
Protocol detail
Measurement of Ocular Cytokine Levels
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Elisa
Il 1ra
Il 1β
Mouse
Muramyl dipeptide
Protein
Corresponding Organization : Portland VA Medical Center
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Variable analysis
independent variables
- Muramyl dipeptide (MDP)
- Mouse recombinant IL-1β
- IL-1Ra protein levels
- IL-1α protein levels
- IL-1β protein levels
- Whole eye homogenates
- Positive control: Not specified
- Negative control: Not specified
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