Western Blot Protein Detection Assay

Protein was harvested and western blot performed as described (21 (link),22 (link)) using the following antibodies: RRM1 (Cell Signaling #8637, RRID:AB_11217623), STIM1 (Sigma-Aldrich S6197, RRID:AB_1079007), ATF4 (Cell Signaling #11815, RRID:AB_2616025), HA (Roche #3F10, RRID:AB_2314622), GAPDH (Origene #TA802519, RRID:AB_2626378), HSC70 (Santa Cruz #sc-7298, RRID:AB_627761), anti-rabbit IgG (Jackson ImmunoResearch Labs #211-032-171, RRID:AB_2339149) and anti-mouse IgG (Jackson ImmunoResearch Labs #115-035-174, RRID:AB_2338512). Further details are in Supplemental Material and Methods.

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Kutschat A.P., Hamdan F.H., Wang X., Wixom A.Q., Najafova Z., Gibhardt C.S., Kopp W., Gaedcke J., Ströbel P., Ellenrieder V., Bogeski I., Hessmann E, & Johnsen S.A. (2021). STIM1 Mediates Calcium-dependent Epigenetic Reprogramming in Pancreatic Cancer. Cancer research, 81(11), 2943-2955.

Publication 2021

STIM1 GAPDH HSC70

Anti igg Antibodies Atf4 Gapdh Mouse Protein Rabbit Rrm1 Stim1 Western blot

Corresponding Organization : Mayo Clinic

Other organizations : Universitätsmedizin Göttingen, University of Göttingen

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Protein expression levels of RRM1, STIM1, ATF4, HA, GAPDH, HSC70

control variables

Not explicitly mentioned

controls

Positive control: None specified
Negative control: None specified

Annotations

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