293T, TE671, D17 and M. dunni cells (Bishop laboratory cell stocks) and U20S and U/R cells (Bacharach laboratory cell stocks) were maintained in DMEM (Invitrogen) supplemented with 10% heat inactivated foetal calf serum (Biosera) and 1% penicillin/streptomycin (Sigma), in a humidified incubator at 37°C and 5% CO2. U/R cells which stably express mCAT-1 [12] (link) were maintained in the presence of 100 ug/ml Zeocin (Invitrogen).
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