Angiotensin II-Induced Muscle Atrophy
Corresponding Organization : Tulane University
Variable analysis
- Delivery of PP2Cα siRNA vs. scrambled siRNA into contralateral Gas muscles
- Infusion of sterile saline (control) vs. 1,000 ng/kg/min AngII
- Muscle weight
- Expression/activity of PP2Cα
- Tissue processing and embedding method
- Muscle cryosectioning procedure
- Animal model (mice)
- Timing of tissue collection (3 days after siRNA electroporation and 4 days after minipump implantation)
- Negative control: Scrambled siRNA
- Positive control: Saline infusion
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