Ovarian CYP27B1 and Ki67 Expression

The expression of CYP27B1 in ovarian tissues was detected using immunohistochemistry, as previously described (48 (link)). Briefly, formalin-fixed paraffin-embedded 4- to 5-μm sections were labeled overnight at 4°C with rabbit anti-CYP27B1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at a dilution of 1:75. The antigen-antibody binding was visualized with HRP-labeled anti-rabbit antibody and 3,3′-diaminobenzidine (DAB) (Envision System-HRP Labeled Polymer Anti-Mouse; Dako, Glostrup, Denmark), followed by hematoxylin counterstaining. The kidney sections served as positive control.
Furthermore, Ki67 immunostaining was performed as described previously (21 (link),49 (link),50 (link)).

Free full text: Click here

BROŻYNA A.A., JÓŹWICKI W., JOCHYMSKI C, & SLOMINSKI A.T. (2014). Decreased expression of CYP27B1 correlates with the increased aggressiveness of ovarian carcinomas. Oncology Reports, 33(2), 599-606.

Publication 2014

Envision System-HRP Labeled Polymer Anti-Mouse

Anti antibody Antibody Antigen Dilution Formalin Hematoxylin Immunohistochemistry Kidney Mouse Ovarian Paraffin Polymer Rabbit Tissues

Corresponding Organization :

Other organizations : Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, University of Tennessee Health Science Center

Top 5 similar protocols

Variable analysis

independent variables

Expression of CYP27B1 in ovarian tissues

dependent variables

Immunohistochemical detection of CYP27B1 expression
Ki67 immunostaining

control variables

Formalin-fixed paraffin-embedded 4- to 5-μm sections
Rabbit anti-CYP27B1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at a dilution of 1:75
HRP-labeled anti-rabbit antibody and 3,3′-diaminobenzidine (DAB) (Envision System-HRP Labeled Polymer Anti-Mouse; Dako, Glostrup, Denmark)
Hematoxylin counterstaining
Kidney sections as positive control

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!