Human alveolar epithelial cells (A549) (ATCC, Manassas, VA) were maintained in Dulbecco's Modified Eagle's Medium (DMEM) (Corning, Manassas, VA), supplemented with 10% Fetal Bovine Serum (FBS) (Thermo Scientific, Rockford, IL) and 1% of antibiotics/antimycotics mixture (Life Technologies, Grand Island, NY) at 37°C, 5% CO2 and 95% relative humidity. Cells were seeded onto 40 mm diameter glass cover slips inside 60 mm petri dishes at a cell density of 3.6 x 104 cells/cm2 and grown to confluence. Based on previous studies which treated lung epithelial or endothelial cells with 0.1 to 100μM Simvastatin [29 (link), 31 (link)–33 (link)], in this study, A549 cells were incubated with 2.5 to 50μM Simvastatin (Cayman Chemical, Ann Arbor, MI) in supplemented media for 16–17 hours prior to each experiment and untreated cells were used as controls.
Protocol detail
Simvastatin treatment of A549 cells
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
A549 cells
Alveolar epithelial cells
Antibiotics
Cayman
Cells
Dishes
Endothelial cells
Fetal bovine serum
Human
Humidity
Lung
Simvastatin
Corresponding Organization : The Ohio State University Wexner Medical Center
Other organizations : The Ohio State University
Top 5 similar protocols
Variable analysis
independent variables
- Simvastatin concentration (2.5 to 50 μM)
- Undefined
- Cell type: Human alveolar epithelial cells (A549)
- Cell culture media: Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% Fetal Bovine Serum (FBS) and 1% antibiotics/antimycotics
- Cell seeding density: 3.6 x 10^4 cells/cm^2
- Cell growth condition: 37°C, 5% CO2, 95% relative humidity
- Incubation time: 16-17 hours
- Positive control: Untreated A549 cells
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!