Plasma TG, LPL Mass and Activity Assay

TG levels in mouse plasma samples were measured using the Serum TG Determination Kit (Millipore-Sigma). LPL mass and activity were measured in plasma samples (collected 2 min after an intravenous injection of 15 U heparin). The plasma samples were adjusted to 1.2 M NaCl and 50 U/mL heparin and stored at –80 °C. LPL mass was measured with a sandwich enzyme-linked immunoassay as described (22 (link)); LPL levels were calculated by linear regression from dilutions that fell within the linear range of the standard curve (from purified mouse LPL). LPL activity was measured with a [³H] triolein substrate using rat serum as a source of APOC2 (22 (link)). Activity was expressed as units of TG hydrolase activity (with 1 mU corresponding to 1 nmol fatty acid release/min).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Song W., Yang Y., Heizer P., Tu Y., Weston T.A., Kim J.R., Munguia P., Jung H., Fong J.L., Tran C., Ploug M., Beigneux A.P., Young S.G, & Fong L.G. (2023). Intracapillary LPL levels in brown adipose tissue, visualized with an antibody-based approach, are regulated by ANGPTL4 at thermoneutral temperatures. Proceedings of the National Academy of Sciences of the United States of America, 120(8), e2219833120.

Publication 2023

Apoc2 Dilutions Enzyme immunoassay Fatty acid Heparin Mouse Nacl Plasma Serum Tg hydrolase Triolein

Corresponding Organization :

Other organizations : University of California, Los Angeles, Rigshospitalet, University of Copenhagen

Top 5 similar protocols

Variable analysis

independent variables

Intravenous injection of 15 U heparin

dependent variables

TG levels in mouse plasma samples
LPL mass
LPL activity

control variables

Plasma samples were adjusted to 1.2 M NaCl and 50 U/mL heparin
Plasma samples were stored at -80 °C
Rat serum as a source of APOC2 for LPL activity measurement

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!