In order to detect the integration of HBV DNA into human genome, targeted HBV DNA fragment capture sequencing was performed. DNA was extracted from tumor tissues and matched non-tumor tissues using GeneReadTM DNA FFPE Kit (QIAGEN, Hilden, Germany). Sequence-capture probes according to eight types (A, B, C, D, E, F, G and H) of HBV genome sequences were produced by MyGenostics (Beijing, China). Detailed targeted HBV DNA fragment capture sequencing procedure was carried out according to a previous report.16 (link) Then, high-throughput viral integration detection (HIVID) method was used for the detection of breakpoints.17 (link) After removal of low-quality reads and duplication reads, sequenced datasets were compared against both HBV and human (hg19) reference sequences using Burrows-Wheeler Aligner (BWA). Integration sites were determined through Clipping Reveals Structure (CREST) analysis.
Protocol detail
Targeted HBV DNA Sequencing for Viral Integration
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : 302 Military Hospital of China, Chinese PLA General Hospital, Beijing Anzhen Hospital, Capital Medical University, Beijing Tsinghua Chang Gung Hospital, Tsinghua University, Second Military Medical University
Top 5 similar protocols
Variable analysis
independent variables
- Eight types (A, B, C, D, E, F, G and H) of HBV genome sequences used for sequence-capture probes
- Detection of HBV DNA integration into the human genome
- Matched non-tumor tissues used as a control for comparison with tumor tissues
- Not explicitly mentioned
- Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!