Anti-HLA Antibody Detection in Transplant Patients

Patient serum was collected before transplantation and was screened for the presence of class I and class II anti-HLA antibodies (HLA abs) of the immunoglobulin G type with a LABScreen Mixed Kit (One Lambda, Canoga Park, CA, USA) according to the manufacturer’s instructions and as previously reported^{9 (link)}. The mean fluorescence intensity (MFI) of the anti-HLA antibodies was adjusted for the background signal using the formula described previously. Samples with an MFI of 500 or more considered to be positive.

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Cao L.Q., Lv M., Xu L.P., Zhang X.H., Chen H., Chen Y.H., Wang F.R., Han W., Sun Y.Q., Yan C.H., Tang F.F., Mo X.D., Liu K.Y., Huang X.J, & Chang Y.J. (2020). Prevalence and risk factors of having antibodies to class I and II human leukocyte antigens in older haploidentical allograft candidates. Scientific Reports, 10, 2367.

Publication 2020

LABScreen Mixed Kit

Anti antibodies Fluorescence Immunoglobulin g Patient Serum Transplantation

Corresponding Organization :

Other organizations : Peking University People's Hospital, Peking University, Center for Life Sciences

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Variable analysis

independent variables

Presence of class I and class II anti-HLA antibodies (HLA abs) of the immunoglobulin G type

dependent variables

Mean fluorescence intensity (MFI) of the anti-HLA antibodies

control variables

Background signal

controls

Samples with an MFI of 500 or more considered to be positive

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