We further explored the potential functions of the identified pleiotropic genes in a comprehensive gene expression data set containing 200 subjects [24 (link)]. The data set was downloaded from Gene Expression Omnibus with the accession number GSE32512. Total RNA was isolated from all samples. Transcriptional profiling was performed based on Illumina Human HT-12 v3 Expression BeadChip. The correlations between gene expression levels and different phenotypes including BMI and insulin activities (insulin resistance and insulin sensitivity) were evaluated [24 (link)]. We assessed the associations between the pleiotropic genes and the BMI and insulin resistance phenotypes to determine whether the pleiotropic genes identified in our study are potentially associated with both obesity and T2D phenotypes in gene expression data.