In stroke patients and a sub-group (n = 18) of healthy subjects, surface electromyography (EMG) of medial gastrocnemius and soleus muscles was recorded with the NeuroFlexor assessment during the whole passive movement from onset until full ankle dorsiflexion.
For EMG recordings, disposable gelled Ag/AgCl electrodes were used. EMG signals were amplified with a Grass LP511 Ac Amplifier (Grass Technologies, Astro-Med, Inc., West Warwick, RI, USA), sampled at 1 kHz using a CED Power1401 (Cambridge Electronic Designs, Cambridge, UK) and rectified. The root mean square of the EMG signal, with a 50 ms sliding window, was computed to generate the EMG amplitude during the whole NeuroFlexor movement from onset until full dorsiflexion of ankle. Data were acquired with Spike2 software (Version 7.12; CED) and analysed off-line using custom-written programmes in MATLAB R2021a (The MathWorks, Inc., Natick, MA, USA).