BDNF was detected with the mouse monoclonal BDNF antibody Mab#9 (anti-BDNF; Kolbeck et al., 1999 (link)) directed against the mature domain of BDNF (Dieni et al., 2012 (link)). For postsynaptic labeling, a rabbit polyclonal antibody against Synaptopodin (“anti-synpo”; #163002; Synaptic Systems), and a guinea pig polyclonal antibody against activity-related cytoskeletal protein (“anti-Arc”; #156005; Synaptic Systems) were used. For labeling of neuropeptides within the MF projection, rabbit polyclonal antibodies against Met-enkephalin (“anti–Met-enk”; Millipore) and Cholecystokinin (anti-CCK; #P06307; Millipore) were applied. Microglia were detected with a rabbit polyclonal antibody against ionized calcium-binding adaptor molecule 1 (“anti-Iba1”; #019-19741; Wako Chemicals). Reelin was detected with a mouse monoclonal Reelin (clone G10) antibody (“anti-Reelin”; #MAB5364; Millipore), while for Calbindin-D28K (CB) labeling, a polyclonal rabbit antibody (“anti-CB”; #CB-38a; Swant) was used. 4′6-Diamidine-2-phenylindol (DAPI, 1:1000, 1 mg/mL solution, ThermoScientific) was used for nuclear counterstaining.
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