Serum BDNF Concentration Measurement Protocol

As described in more detail elsewhere,^{34 (link)} serum was separated immediately after blood draw and stored (at −85 °C) until assay. Serum BDNF concentrations were determined using the Emax Immuno Assay system from Promega according to the manufacturer’s protocol (Madison, WI, USA). Absorbency was read in duplicate using a Bio-Rad Benchmark microplate reader (Hercules, CA, USA) at 450 nm. The coefficients of variance ranged between 2.9 and 8.1%. In total, 2498 (97.5%) of the subjects included in our sample had a BDNF value available. A previous NESDA paper^{3 (link)} showed that anti-depressant-free currently depressed participants had lower BDNF levels than both HCs and their medicated currently depressed peers.

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Verduijn J., Milaneschi Y., Schoevers R.A., van Hemert A.M., Beekman A.T, & Penninx B.W. (2015). Pathophysiology of major depressive disorder: mechanisms involved in etiology are not associated with clinical progression. Translational Psychiatry, 5(9), e649-.

Publication 2015

Emax Immuno Assay system Benchmark microplate reader

Assay Blood Immuno assay Promega Serum

Corresponding Organization : Amsterdam Neuroscience

Other organizations : University Medical Center Groningen, University of Groningen, Leiden University Medical Center

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Serum BDNF concentrations

control variables

Serum was separated immediately after blood draw and stored (at −85 °C) until assay
Serum BDNF concentrations were determined using the Emax Immuno Assay system from Promega according to the manufacturer's protocol
Absorbency was read in duplicate using a Bio-Rad Benchmark microplate reader at 450 nm
The coefficients of variance ranged between 2.9 and 8.1%

positive controls

Not mentioned

negative controls

Not mentioned

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