Analyzing Immune Cells Post-Myocardial Infarction

Mice were sacrificed at 1 day and 7 days after MI. The blood, spleen, bone marrow and infarcted myocardium were collected and made into single-cell suspensions for flow cytometry, as previously described26 (link)52 (link). The cells were stained with a mixture of antibodies (anti-CD11b-APC, anti-Gr-1-PerCP-Cy5.5, anti-Ly6C-PE; BD Biosciences). Data were acquired using an LSRII flow cytometer (BD Biosciences) and were analyzed with FlowJo7 software (Tree Star, Inc.).

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Deng L., Hong T., Lin J., Ding S., Huang Z., Chen J., Jia J., Zou Y., Wang T.C., Yang X, & Ge J. (2015). Histamine deficiency exacerbates myocardial injury in acute myocardial infarction through impaired macrophage infiltration and increased cardiomyocyte apoptosis. Scientific Reports, 5, 13131.

Publication 2015

anti-CD11b-APC anti-Gr-1-PerCP-Cy5.5 anti-Ly6C-PE LSRII flow cytometer FlowJo7 software

Antibodies Blood Bone marrow Cd11b Cells Cy5 5 Flow cytometry Mice Myocardium Spleen Tree

Corresponding Organization :

Other organizations : The First Affiliated Hospital, Sun Yat-sen University, Zhongshan Hospital, Fudan University, Sun Yat-sen University, Columbia University

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Variable analysis

independent variables

Time after MI (1 day, 7 days)

dependent variables

Cell populations in blood, spleen, bone marrow, and infarcted myocardium as measured by flow cytometry

control variables

Antibodies used for staining (anti-CD11b-APC, anti-Gr-1-PerCP-Cy5.5, anti-Ly6C-PE)
Flow cytometry instrument (LSRII flow cytometer)
Flow cytometry data analysis software (FlowJo7)

controls

Positive control: Not specified
Negative control: Not specified

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