Synthesis and Characterization of Prostate Cancer Compounds

Galeterone, DHT, VNPT-178, VNLG-74A, and abiraterone were synthesized in-house as previously described [7 (link), 35 (link)]. Enzalutamide (a.k.a. MDV3100, #SRP016825m) was purchased from Sequoia Research Products (Pangbourne, UK). Methyltrienolone (a.k.a. R1881, #965M935) was purchased from OChem Incorporation (Des Plaines, IL). Z-VAD-FMK (FMK001) was purchased from R&D Systems (Minneapolis, MN). Etoposide (E1383) was purchased from Sigma (St. Louis, MO). Calpeptin (SC-202516) was purchased from Santa Cruz Biotechnology (Dallas, TX). All compounds were solubilized in 95% ethanol (Pharmco-AAPER; Brookfield, CT) or ultrapure DMSO (AmericanBio; Natick, MA).