Cultivation and Characterization of L. infantum Promastigotes

L. infantum (MCAN/BR/2008/OP46) promastigotes, which were isolated from an infected dog at Governador Valadares, MG, Brazil, and characterized by molecular techniques and hamster infection^{23 (link),24 (link)}. They were maintained at 26 °C in NNN-LIT medium (Sigma) supplemented with 20% heat-inactivated fetal calf serum (Criprion Biotech) and 40 μg gentamicin ml⁻¹ (Schering-Plough). For all assays, promastigotes in the stationary phase of growth (5–6 days) were washed twice in PBS (pH 7.2) at 1900 x g for 13 min at room temperature and counted using a hemocytometer.

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Wardini A.B., Pinto-da-Silva L.H., Nadaes N.R., Nascimento M.T., Roatt B.M., Reis A.B., Viana K.F., Giunchetti R.C, & Saraiva E.M. (2019). Neutrophil properties in healthy and Leishmania infantum-naturally infected dogs. Scientific Reports, 9, 6247.

Publication 2019

gentamicin

Assays Fetal calf serum Gentamicin Hamster

Corresponding Organization :

Other organizations : Universidade Federal do Rio de Janeiro, Universidade Federal Rural do Rio de Janeiro, Universidade Federal de Ouro Preto, Universidade Federal de Minas Gerais

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Variable analysis

independent variables

Leishmania infantum (MCAN/BR/2008/OP46) promastigotes

dependent variables

Not explicitly mentioned

control variables

Culture temperature of 26 °C
NNN-LIT medium (Sigma) supplemented with 20% heat-inactivated fetal calf serum (Criprion Biotech) and 40 μg gentamicin ml^-1 (Schering-Plough)
Promastigotes in the stationary phase of growth (5–6 days)
Washing of promastigotes twice in PBS (pH 7.2) at 1900 x g for 13 min at room temperature

controls

No positive or negative controls were explicitly mentioned.

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