We used the monoclonal AQP4-specific antibody E5415A [23 (link)], termed “AQP4-abs” throughout the manuscript, and control mouse IgG (Sigma, Vienna, Austria), both in a concentration of 1 mg/ml in phosphate-buffered saline (PBS). The T-cell lines specific for myelin basic protein (MBP) and aquaporin 4 (AQP4268–285) were essentially produced as described [57 (link)].
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