Immunoblotting Antibody Protocol

Immunoblotting was performed as described [74 (link), 75 (link)]. The antibodies used were anti-Flag (Sigma-Aldrich), polyclonal anti-YOD1 (Proteintech, Rosemont, IL), monoclonal and polyclonal anti-p53 (Cell Signaling Technology, Beverly, MA and Santa Cruz Biotechnology respectively), anti-Myc, anti-PUMA, anti-BAX, and anti-MDM2 antibodies (Santa Cruz Biotechnology), anti-K48 ubiquitin and β-actin (Cell Signaling Technology), anti-HA (Abcam), and horseradish peroxidase-conjugated secondary antibodies (Cell Signaling Technology).

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Pei H.Z., Peng Z., Zhuang X., Wang X., Lu B., Guo Y., Zhao Y., Zhang D., Xiao Y., Gao T., Yu L., He C., Wu S., Baek S.H., Zhao Z.J., Xu X, & Chen Y. (2023). miR-221/222 induce instability of p53 By downregulating deubiquitinase YOD1 in acute myeloid leukemia. Cell Death Discovery, 9, 249.

Publication 2023

anti-Flag anti-p53 anti-Myc anti-PUMA anti-BAX anti-HA horseradish peroxidase-conjugated secondary antibodies

Actin Anti antibodies Antibodies Horseradish peroxidase Mdm2 Puma Ubiquitin

Corresponding Organization : University of Oklahoma Health Sciences Center

Other organizations : The Seventh Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels of Flag, YOD1, p53, Myc, PUMA, BAX, MDM2, K48 ubiquitin, and β-actin

control variables

None explicitly mentioned

controls

Positive controls: None specified
Negative controls: None specified

Annotations

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