Western Blot Analysis of EMT Markers

The Western blot was processed as previously described.12 (link) The primary antibodies used in the present study were listed below: wnt3a (1:1000, ab19925, Abcam), β-catenin (1:1000, 51067-2-AP, Proteintech), Vimentin (1:1000, 10366-1-AP, Proteintech), E-cadherin (1:500, 20874-1-AP, Proteintech), N-cadherin (1:500, 22018-1-AP, Proteintech), Fibronectin (1:500, 15613-1-AP, Proteintech), Snail (1:500, 13099-1-AP, Proteintech) and GAPDH (1:2000, 60004-1-Ig, Proteintech). The blots were developed using the ECL substrate (Beyotime, China) and photographed using the imaging system (Bio-Rad, CA, USA).

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Li H., Cui X., Hu Q., Chen X, & Zhou P. (2019). CLK3 Is A Direct Target Of miR-144 And Contributes To Aggressive Progression In Hepatocellular Carcinoma. OncoTargets and therapy, 12, 9201-9213.

Publication 2019

wnt3a ab19925 β-catenin Vimentin E-cadherin N-cadherin Fibronectin ECL substrate imaging system

Antibodies E cadherin Fibronectin 1 Gapdh N cadherin Snail Vimentin Western blot β catenin

Corresponding Organization : First Affiliated Hospital of Zhengzhou University

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Variable analysis

independent variables

Wnt3a (1:1000, ab19925, Abcam)
β-catenin (1:1000, 51067-2-AP, Proteintech)
Vimentin (1:1000, 10366-1-AP, Proteintech)
E-cadherin (1:500, 20874-1-AP, Proteintech)
N-cadherin (1:500, 22018-1-AP, Proteintech)
Fibronectin (1:500, 15613-1-AP, Proteintech)
Snail (1:500, 13099-1-AP, Proteintech)

dependent variables

Not explicitly mentioned

control variables

GAPDH (1:2000, 60004-1-Ig, Proteintech)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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