Magnetic resonance imaging (MRI) and MR spectroscopy (MRS) were used to monitor brain tumor growth as described previously (Connolly et al., 2017 (link)). All MRI scans were performed using a Bruker BioSpec 70/30USR Advance III 7T horizontal bore MR scanner (Bruker Biospin MRI) equipped with a BGA12S gradient system and interfaced to a Bruker Paravision 5.1 console. An MR compatible small-animal monitoring and gating system (SA Instruments Inc.) was used to monitor animal respiration rate and body temperature which was maintained at 37–38.5°C using a warm water bath circulation. LCModel package (version 6.3-0G; LCModel Inc.) (Provencher, 2001 (link)) was used for quantification of the MRS data. The reliability of the major biochemical entities was estimated in the Cramér–Rao lower bounds (CRLB) from the LCModel analysis. LCModel package was used for quantification of the MRS data. The primary biochemical entities' reliability was estimated in the CRLB from the LC Model analysis.