Normal human astrocytes (NHA) were purchased from ScienceCell Research Laboratories (USA) and cultured in Astrocyte Medium (ScienCell Research Laboratories, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, USA) and 1% penicillin/streptomycin (Welgene, Korea). GBM patient-derived GSC11 and GSC23 were obtained from the University of Texas MD Anderson Cancer Center.22 (link) GSCs were maintained using neurobasal medium (NBE, serum-free Neurobasal media supplemented with basic FGF and EGF). NBE conditions were as follows: Dulbecco’s modified Eagle’s medium (DMEM)/F12 (Welgene) was supplemented with 2% B27 (50×) (Gibco), 1% penicillin/streptomycin (Welgene), epidermal growth factor (EGF; 20 ng/mL; R&D Systems, USA), and basic fibroblast growth factor (bFGF; 20 ng/mL; R&D Systems). Serum-free conditions were as follows: Dulbecco’s modified Eagle’s medium (DMEM)/F12 (Welgene) was supplemented with 5% UltraGROTM–Advanced Cell Culture Supplement (AventaCell) and 1% penicillin/streptomycin (Welgene).
Protocol detail
Culturing Glioblastoma Stem Cells and Normal Astrocytes
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Astrocyte
Basic fibroblast growth factor
Cancer
Cell culture
Eagle
Epidermal growth factor
Human
Patient
Penicillin
Serum
Serum free media
Streptomycin
Supplement
Corresponding Organization :
Other organizations : Chonnam National University, Mokpo National University, Korea Basic Science Institute, Korea Polar Research Institute, Cheongju University, Sungkyunkwan University
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Variable analysis
independent variables
- Normal human astrocytes (NHA) vs. glioblastoma (GBM) patient-derived GSC11 and GSC23 cell lines
- Not explicitly mentioned
- Cell culture conditions for NHA: Astrocyte Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin
- Cell culture conditions for GSC11 and GSC23: Neurobasal medium (NBE) supplemented with basic FGF and EGF
- Serum-free conditions: Dulbecco's modified Eagle's medium (DMEM)/F12 supplemented with 5% UltraGRO-Advanced Cell Culture Supplement and 1% penicillin/streptomycin
- Positive control: Not mentioned
- Negative control: Not mentioned
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