ARPE-19 real time migration was assessed using an xCELLigence RTCA DP Instrument (F. Hoffmann-La Roche SA, Basel, Switzerland), using the specific CIM-Plates (Agilent, Santa Clara, CA, USA), electronically integrated Boyden Chambers. In particular, the xCELLigence RTCA DP Instrument registers, in real time, impedance values that are related to cell migration, and then converts them into an adimensional parameter, that is the “Cell Index” (CI). For migration experiments, ARPE-19 cells were treated for 24 h in 6 wells in complete DMEM/F-12 medium with different concentrations of Nutlin-3 or LIPO0.5-NUT, using untreated cells as controls. After treatments, cells were starved from FBS for 2 h before being detached, accurately counted and then seeded in the upper chamber of the CIM-Plates (4 × 104 cells/well), following the manufacturer’s instructions. Finally, plates were inserted into the instruments and migration data were recorded every 5 min.