We also used the ARRIVE reporting guidelines.21 (link) First, mice were preconditioned by one time IP injection of 20 mg/kg of busulfan (Millipore Sigma, B2635-25G).22 (link) The next day, 2M laNK92-nLuc cells were injected IP into female hIL-2 NOG aged 4–6 weeks (model# 13 440-F), hIL-15 NOG (model# 13 683-F), and CIEA NOG (model# NOG-F) mice (Taconic Biosciences). One CIEA NOG mouse received daily injection of hIL-2 (50K UI) for five consecutive days, whereas the second one did not receive any hIL-2. Before imaging, mice were anesthetized by 2% isoflurane and then injected IP with furimazine (Promega) (2 µL furimazin in 198 µL of DPBS). The BLI in each mouse was detected within 30 s of furimazine injection with acquisition time of 60 s using an IVIS Lumina III Imaging System. To determine the fold change in mass of laNK92-nLuc cells over time, the total BLI of the mouse body on day 35 was divided by the total BLI of the same mouse on day 1.
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In Vivo Bioluminescent Imaging of laNK92-nLuc Cells
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Other organizations : Rutgers, The State University of New Jersey, Taconic (United States), St. Michael's Hospital, St Michaels Hospital
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Variable analysis
independent variables
- Busulfan (20 mg/kg) administered by IP injection
- HIL-2 (50K UI) administered by daily IP injection for five consecutive days
- Fold change in mass of laNK92-nLuc cells over time (measured by BLI on day 35 divided by day 1)
- Mouse strains: female hIL-2 NOG, hIL-15 NOG, and CIEA NOG (Taconic Biosciences)
- Age of mice: 4-6 weeks
- Route of cell injection: IP
- Cell line: laNK92-nLuc
- Anesthesia: 2% isoflurane
- Bioluminescence imaging: Furimazine injection followed by imaging within 30 seconds, acquisition time of 60 seconds using IVIS Lumina III Imaging System
- Positive control: One CIEA NOG mouse received daily injection of hIL-2 (50K UI) for five consecutive days
- Negative control: One CIEA NOG mouse did not receive any hIL-2
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