Endothelial Barrier Regulation by Flaviviral NS1

HPMEC monolayers grown on a 24-well Transwell polycarbonate membrane system (0.4 μM, 6.5 mm insert; Corning) were exposed to 10 μg/mL of DENV-2 NS1 and then treated with different concentrations of FR or FR-S (0.12, 0.06, or 0.03 μg/mL). As positive controls, HPMECs with DENV-2 NS1 were left untreated. As basal controls, HPMECs were left untreated. Trans-Endothelial Electrical Resistance (TEER) was measured using a Volt/Ohm Meter (EVOM2, World Precision Instruments), as described previously (Puerta-Guardo et al., 2016 (link)). Relative TEER was calculated as the ratio between resistance values obtained in the treated conditions and basal controls. For the ZIKV NS1 assay, we tested FR and FR-S on HBMEC monolayers treated with 10 μg/mL ZIKV NS1. HPMECs were chosen for evaluation of DENV-2 NS1 due to leakage in the lung during severe dengue (pleural effusion), while HBMECs were tested with ZIKV NS1 due to brain pathology seen in severe ZIKV infections.

Free full text: Click here

de Sousa F.T., Biering S.B., Patel T.S., Blanc S.F., Camelini C.M., Venzke D., Nunes R.J., Romano C.M., Beatty P.R., Sabino E.C, & Harris E. (2022). Sulfated β-glucan from Agaricus subrufescens inhibits flavivirus infection and nonstructural protein 1-mediated pathogenesis. Antiviral research, 203, 105330.

Publication 2022

Transwell polycarbonate membrane system EVOM2

Assay Brain Electrical resistance Endothelial Lung Membrane Pleural effusion Polycarbonate Seen Severe dengue Zikv Zikv infections

Corresponding Organization : University of California, Berkeley

Other organizations : Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Universidade Federal de Santa Catarina

Top 5 similar protocols

Variable analysis

independent variables

Concentration of FR or FR-S (0.12, 0.06, or 0.03 μg/mL)

dependent variables

Trans-Endothelial Electrical Resistance (TEER)

control variables

HPMEC monolayers grown on a 24-well Transwell polycarbonate membrane system (0.4 μM, 6.5 mm insert; Corning)
Exposure to 10 μg/mL of DENV-2 NS1

positive controls

HPMECs with DENV-2 NS1 left untreated

negative controls

HPMECs left untreated

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!