Transient Transfection of CHO Cells

Chinese hamster ovary (CHO) cells (ATCC Cat# CCL-61, RRID:CVCL_0214; American Type Culture Collection) were cultured in Ham’s F12 media (10-080, Corning/Cellgro) supplemented with 10% fetal bovine serum (Genesee Scientific Cat#25-514H). Transient transfection assays were carried out using the Lipofectamine 2000 reagent (Thermo Fisher Scientific, Inc., Cat#11668019). Cells were grown to 90% confluency, and transfections were performed according to the manufacturer’s instructions and according to the protocols outlined in.
^{24 (link)}^,^{25 (link)} The chick
N-cadherin-expressing (pCIG-N-cadherin) and empty (pCIG) vectors were gifts from Dr. Marianne Bronner (California Institute of Technology).

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Halmi C.A., Wu C.Y, & Taneyhill L.A. (2022). Neural crest cell-placodal neuron interactions are mediated by Cadherin-7 and N-cadherin during early chick trigeminal ganglion assembly. F1000Research, 11, 741.

Publication 2022

Ham’s F12 media Lipofectamine 2000 reagent

Assays Cadherin Cells Chinese hamster ovary cells Cultured media Fetal bovine serum Gifts Lipofectamine 2000 Transfections Transient Vectors

Corresponding Organization :

Other organizations : University of Maryland, College Park, United States Food and Drug Administration

Top 5 similar protocols

Variable analysis

independent variables

Transfection of chick N-cadherin-expressing (pCIG-N-cadherin) and empty (pCIG) vectors

dependent variables

Not explicitly mentioned

control variables

Chinese hamster ovary (CHO) cells (ATCC Cat# CCL-61, RRID:CVCL_0214; American Type Culture Collection)
Ham's F12 media (10-080, Corning/Cellgro) supplemented with 10% fetal bovine serum (Genesee Scientific Cat#25-514H)
Lipofectamine 2000 reagent (Thermo Fisher Scientific, Inc., Cat#11668019)
Cells were grown to 90% confluency

positive controls

Chick N-cadherin-expressing (pCIG-N-cadherin) vector

negative controls

Empty (pCIG) vector

Annotations

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