Lentivirus-mediated ABIN1 Knockdown

Lentiviruses containing the ABIN1 shRNA (LV-shABIN1) for ABIN1 knockdown and control shRNA (LV-Scramble) were supplied by Genechem (Shanghai, China). Two weeks prior to the establishment of the MCAO/R model, the right cortex of rat was injected with the lentiviruses. The injection sites were as follows: site 1, A-P 1.0 mm; M-L −2.0 mm; D-V −1.2 mm, and site 2, A-P −3.0 mm; M-L −1.5 mm; D-V −1.2 mm [34 (link)]. A total of 2.5 μl of LV-shABIN1 or LV-Scramble were injected into each site.

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Zhou X., Lu W., Wang Y., Li J, & Luo Y. (2020). A20-Binding Inhibitor of NF-κB 1 Ameliorates Neuroinflammation and Mediates Antineuroinflammatory Effect of Electroacupuncture in Cerebral Ischemia/Reperfusion Rats. Evidence-based Complementary and Alternative Medicine : eCAM, 2020, 6980398.

Publication 2020

LV-Scramble

Cortex Injection sites Lentiviruses Shrna

Corresponding Organization : Chongqing Medical University

Other organizations : Second Affiliated Hospital of Chongqing Medical University, Dalian Medical University

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Variable analysis

independent variables

Injection of LV-shABIN1 lentivirus
Injection of LV-Scramble lentivirus

dependent variables

Establishment of the MCAO/R model

control variables

Injection sites: site 1 (A-P 1.0 mm; M-L −2.0 mm; D-V −1.2 mm) and site 2 (A-P −3.0 mm; M-L −1.5 mm; D-V −1.2 mm)
Volume of lentivirus injected: 2.5 μl for each site

controls

Positive control: LV-shABIN1 lentivirus
Negative control: LV-Scramble lentivirus

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