CSF miR-142-3p and IL-1β Analysis

CSF was collected at the time of diagnosis, during hospitalization, by lumbar puncture (LP) for medical purpose. No corticosteroids or disease modifying therapies were administered before LP. Cell-free CSF samples were stored at −80 °C and later analyzed [6 (link)]. Briefly, the CSF miR-142-3p levels were detected by quantitative real-time PCR (QIAGEN, Hilden, Germany) and normalized to miR-204-5p [6 (link)]. Samples were analyzed in duplicate. CSF concentrations of IL-1β were determined by using a Bio-Plex multiplex cytokine assay (Bio-Rad Laboratories, Hercules, CA, USA) and expressed in picograms/milliliter (pg/mL) according to the standard curve. Samples were analyzed in triplicate.