Lung specimens were fixed in formaldehyde solution 4%, buffered, pH 6.9 (Merck, Germany). Lung tissue samples were dehydrated and were embedded in paraffin (Merck, Germany) and sectioned into 5 μm thickness slices with 25 μm intervals. A usual hematoxylin and eosin (H&E) staining was performed for histological assessment. Five sections from each corpse were used for evaluations. Two histopathologists, blinded to the groups, evaluated the sections. The sections were visible under a light microscope (LABOMED, Labo America, Inc. USA). A digital camera (LABOMED, USA) was used to take histophotographs. Total histopathological scoring was done according to the following parameters: hemorrhage, thickened wall, inflammatory cells infiltration, and fibrotic changes with a score of None = 0, Mild = 1, Moderate = 2, and Severe = 3 (Gibson-Corley et al. 2013 (link)).
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Histopathological Evaluation of Lung Tissue
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Camera
Cells
Corpse
Embedded paraffin
Eosin
Fibrotic
Formaldehyde solution
Hematoxylin
Hemorrhage
Inflammatory
Lung
Microscope
Tissue
Visible light
Corresponding Organization :
Other organizations : Iranian Legal Medicine Organization, Tehran University of Medical Sciences
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Variable analysis
dependent variables
- Histopathological scoring
- Hemorrhage
- Thickened wall
- Inflammatory cells infiltration
- Fibrotic changes
- Lung specimens were fixed in formaldehyde solution 4%, buffered, pH 6.9 (Merck, Germany)
- Lung tissue samples were dehydrated and were embedded in paraffin (Merck, Germany)
- Sections were 5 μm thickness with 25 μm intervals
- Hematoxylin and eosin (H&E) staining was used for histological assessment
- Sections were evaluated by two histopathologists, blinded to the groups
- Sections were visible under a light microscope (LABOMED, Labo America, Inc. USA)
- A digital camera (LABOMED, USA) was used to take histophotographs
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