Vascular Smooth Muscle Cell Oxidative Stress

ROS levels were measured in VSMCs that had been pre-treated with NoxA1ds (Nox1 inhibitor), GKT137831 (Nox1/4 inhibitor) and 4-Phenylbutyric acid (4-PBA) (ER stress inhibitor) and stimulated with Ang II. Lucigenin-enhanced chemiluminescence was used to detect NADPH-dependent superoxide anion (O₂^-) production in VSMC16 (link). Hydrogen peroxide (H₂O₂) levels were assessed by Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit.

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Camargo L.L., Harvey A.P., Rios F.J., Tsiropoulou S., de Oliveira Silva R.D., Cao Z., Graham D., McMaster C., Burchmore R.J., Hartley R.C., Bulleid N., Montezano A.C, & Touyz R.M. (2018). Vascular Nox compartmentalization, protein hyperoxidation and ER stress response in hypertension. Hypertension (Dallas, Tex. : 1979), 72(1), 235-246.

Publication 2018

4 pba 4 phenylbutyric acid Assay Chemiluminescence Gkt137831 Hydrogen peroxide Lucigenin Nadph Peroxidase Superoxide anion

Corresponding Organization :

Other organizations : Cardiovascular Institute of the South, Universidade de São Paulo, Institute of Molecular and Cell Biology, University of Glasgow, Institute of Infection and Immunity

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Variable analysis

independent variables

NoxA1ds (Nox1 inhibitor)
GKT137831 (Nox1/4 inhibitor)
4-Phenylbutyric acid (4-PBA) (ER stress inhibitor)
Ang II (angiotensin II)

dependent variables

ROS (Reactive Oxygen Species) levels in VSMCs (Vascular Smooth Muscle Cells)
NADPH-dependent superoxide anion (O2-) production in VSMC16
Hydrogen peroxide (H2O2) levels

control variables

VSMC16 cells

positive controls

No information provided

negative controls

No information provided

Annotations

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