The LI-6400XT portable photosynthesis measurement system was used to determine the photosynthesis of male and hermaphroditic O. fragrans at the full flowering stage according to the instructions. Four plants of each sex were selected, and 8 healthy and mature leaves of each plant were selected for measurement, and the net photosynthetic rate (Pn), water use efficiency (WUE) and other data were recorded.
Total RNA of male and hermaphroditic pistils were extracted with RNAprep Pure Plant Plus Kit (TIANGEN Biotech, Beijing) according to the manufacturer’s instructions. Then, 5 μg RNA was reversed transcribed by Evo M-MLV RT Premix for qPCR (Accurate Biotechnology, Hunan) for cDNA synthesis. SYBR Green Premix Pro Taq HS qPCR Kit (Accurate Biotechnology, Hunan) was used for quantitative real-time PCR (qRT-PCR) experiments with OfACT as a reference [69 (link)]. qRT-PCR was performed using ABI StepOnePlus Systems (Applied Biosystems, USA), the reaction steps were as follows: 95 °C for 30 s, followed by 40 cycles of 95 °C for 5 s and 60 °C for 30 s. All primers used in this experiment were listed in TableS3 .
Total RNA of male and hermaphroditic pistils were extracted with RNAprep Pure Plant Plus Kit (TIANGEN Biotech, Beijing) according to the manufacturer’s instructions. Then, 5 μg RNA was reversed transcribed by Evo M-MLV RT Premix for qPCR (Accurate Biotechnology, Hunan) for cDNA synthesis. SYBR Green Premix Pro Taq HS qPCR Kit (Accurate Biotechnology, Hunan) was used for quantitative real-time PCR (qRT-PCR) experiments with OfACT as a reference [69 (link)]. qRT-PCR was performed using ABI StepOnePlus Systems (Applied Biosystems, USA), the reaction steps were as follows: 95 °C for 30 s, followed by 40 cycles of 95 °C for 5 s and 60 °C for 30 s. All primers used in this experiment were listed in Table
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