Immunohistochemistry was performed as previously described10 (link). The following antibodies were used: BMP2 rabbit polyclonal (1:500), OCN rabbit polyclonal (1:200), VEGF-A rabbit monoclonal (1:300), and horseradish peroxidase (HRP)-conjugated secondary antibodies (Severicebio), mouse IgG1-FITC isotype control (1:200, Santa Cruz), Rabbit IgG monoclonal isotype control (1:200, Abcam).
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