Cellular [Ca2+]i and electrophysiological methods are described in the Online Supplement and were used to tune our model and for validation. Table 1 shows key changes made in our new human atrial model vs. our ventricular myocyte model, 12 (link) to account for ionic remodeling in cAF, and to simulate the effects of β-adrenergic and cholinergic stimulation. Further details are in Online Supplement , including formulation of IKur block by AVE0118.
Model differential equations were implemented in Matlab (Mathworks Inc., Natick, MA, USA) and solved numerically using a variable order solver (ode15s). APDs were obtained after pacing digital cells at indicated frequencies at steady-state. APD was measured as the interval between AP upstroke and 90% repolarization level (APD90).
Model differential equations were implemented in Matlab (Mathworks Inc., Natick, MA, USA) and solved numerically using a variable order solver (ode15s). APDs were obtained after pacing digital cells at indicated frequencies at steady-state. APD was measured as the interval between AP upstroke and 90% repolarization level (APD90).
