The MDA-MB-231, SUM149 and SUM159 cell lines (5x104 cells/well) were seeded in 6-well plates and treated with GO (IC50: 3.78, 1.85 and 1.60 mmol/l, respectively) for 24 h. Next, the cells were collected and stored in pre-cooled alcohol overnight at 4˚C, then stained with PI (Shanghai Yeasen Biotechnology, Co., Ltd.) for 15 min in the dark at 4˚C. The samples were tested using a Guava EasyCyte Plus flow cytometer (Merck KGaA) and FlowJo VX (Becton-Dickinson and Company) was used to analyze the results. The test was repeated three times.
Protocol detail
Cell Cycle Analysis of Cancer Cell Lines
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Sichuan University, West China Hospital of Sichuan University
Top 5 similar protocols
Variable analysis
independent variables
- GO (IC50: 3.78, 1.85 and 1.60 mmol/l, respectively)
- Cell death/apoptosis
- Cell lines (MDA-MB-231, SUM149 and SUM159)
- Cell density (5x10^4 cells/well)
- Treatment duration (24 h)
- Staining with PI
- Flow cytometry analysis
- Negative control: Untreated cells
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!