Cell Viability and Proliferation Assay

Cells were plated in quadruplicate (2000 cells/well) in a 96-well plate for the CyQuant assay with the CyQuant reagent (Thermo Fisher Scientific) as previously described [25 (link)]. Cell viability was assessed by trypan blue staining [25 (link)]. Population doubling times were calculated using a population doubling time online calculator (http://www.doubling-time.com/compute.php) based on the reading from CyQuant and viability assays.

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Khatiwala R.V., Zhang S., Li X., Devejian N., Bennett E, & Cai C. (2018). Inhibition of p16INK4A to Rejuvenate Aging Human Cardiac Progenitor Cells via the Upregulation of Anti-oxidant and NFκB Signal Pathways. Stem cell reviews, 14(4), 612-625.

Publication 2018

CyQuant reagent

Assays Cell viability Cells Trypan blue

Corresponding Organization :

Other organizations : Albany Medical Center Hospital

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Variable analysis

independent variables

Cell plating density (2000 cells/well)

dependent variables

Cell viability
Population doubling times

control variables

Quadruplicate cell plating
CyQuant reagent (Thermo Fisher Scientific)
Trypan blue staining

controls

Positive control: None specified
Negative control: None specified

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