Immunohistochemical staining of testicular tissue samples from mouse, monkey and human was carried out as described previously (Mayer et al. 2016 (link)). For each species a minimum of four and up to 14 different sections were examined. Polyclonal rabbit anti-NLRP3 IgG (R30750, NSJ Bioreagents, San Diego, CA, USA) was used as primary antibody to stain for NLRP3. Negative controls consisted of omission of the primary antibody, of incubation with rabbit IgG or non-immune serum instead of the primary antiserum. Sections were counterstained with hematoxylin and visualized using a Leica DM2500 microscope equipped with a DMC2900 CMOS camera or an Zeiss Axiovert microscope with an Insight Camera (18.2 Color Mosaik) and Spot advanced software 4.6 (SPOT Imaging Solutions, Sterling Heights, MI, USA).
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Immunohistochemical Analysis of NLRP3 in Testis
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Anti igg
Antibody
Antiserum
Cmos
Hematoxylin
Human
Microscope
Monkey
Mouse
Rabbit
Stain
Staining tissue
Tissue
Corresponding Organization : Ludwig-Maximilians-Universität München
Other organizations : Oregon National Primate Research Center, German Primate Center, University of Turku
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Variable analysis
independent variables
- Species (mouse, monkey, human)
- NLRP3 expression (immunohistochemical staining)
- Number of sections examined (minimum of 4, up to 14 for each species)
- Primary antibody (polyclonal rabbit anti-NLRP3 IgG)
- Negative controls: omission of primary antibody, incubation with rabbit IgG or non-immune serum
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