Apoptosis was performed in coverslips of ARPE-19, RF/6A, RGCs culture and mice retinal sections using in situ cell death detection kit with TMR Red according to the manufacturer instructions (Roche, West Sussex, UK) (Roche #12 156 792 910) [72 (link),73 (link),89 (link)].
Sections containing the optic nerve head were selected for TUNEL assay TMR Red according to protocols in the manufacturer’s instructions (Roche, West Sussex, UK). Nuclei were labelled with DAPI (40, 6-diamidino-2-phenylindole, Sigma-Aldrich) and images were photographed using a fluorescence and confocal microscope (Axio Imager M1 and LSM800, Zeiss, Oberkochen, Germany).
Sections containing the optic nerve head were selected for TUNEL assay TMR Red according to protocols in the manufacturer’s instructions (Roche, West Sussex, UK). Nuclei were labelled with DAPI (40, 6-diamidino-2-phenylindole, Sigma-Aldrich) and images were photographed using a fluorescence and confocal microscope (Axio Imager M1 and LSM800, Zeiss, Oberkochen, Germany).
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