Comprehensive Western Blot Analysis

Western blot was performed as described before (22 (link)). Primary antibodies were used as follows: Bcl2 (1:200, Cell Signalling Technology (Frankfurt am Main, Germany)), BAX(1:1000, Cell Signalling Technology), c-Jun(1:100, Santa Cruz Biotechnology (Dallas, USA)), p-c-Jun(1:100, Santa Cruz Biotechnology), Lamin B1 (0.1 µg/ml, Abcam). β-actin(1:5000, Sigma Aldrich) and GAPDH (1:5000, Sigma Aldrich) act as the internal control. Images were acquired by FUSION FX (Vilber Lourmat Deutschland GmbH, Weinheim, Germany).

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Shi J., Yang X., Kang Q., Lu J., Denzinger M., Kornmann M, & Traub B. (2022). JNK inhibitor IX restrains pancreatic cancer through p53 and p21. Frontiers in Oncology, 12, 1006131.

Publication 2022

Bcl2 BAX c-Jun p-c-Jun Lamin B1 β-actin GAPDH FUSION FX

Actin Antibodies Bcl2 C jun Gapdh Lamin b1 Western blot

Corresponding Organization : University Hospital Ulm

Other organizations : Nanjing Drum Tower Hospital

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Variable analysis

independent variables

Primary antibodies used: Bcl2, BAX, c-Jun, p-c-Jun, Lamin B1, β-actin, GAPDH

dependent variables

Protein expression levels of Bcl2, BAX, c-Jun, p-c-Jun, Lamin B1, β-actin, GAPDH

control variables

Western blot protocol as described before (22)
Protein loading control: β-actin and GAPDH

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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