Immune Cell Profiling of Murine Kidney

Right kidney was harvested and immediately processed for flow cytometric analysis. The following antibodies were used: anti CD45-V450, Ly6G APC-Cy7, B220 PE-Cy7, CD3 FITC, CD4 PE, CD8 PerCP-Cy5.5 (ThermoFisher Scientific, Waltham).^{42 (link),43} Details are reported in Suppl. Methods.

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Federti E., Matte A., Recchiuti A., Garello F., Ghigo A., El Nemer W., Terreno E., Amoresano A., Mattoscio D., Turrini F., Lebouef C., Janin A., Pantaleo A., Russo R., Marin M., Iatcencko I., Riccardi V., Siciliano A., Iolascon A., Brugnara C, & De Franceschi L. (2023). In Humanized Sickle Cell Mice, Imatinib Protects Against Sickle Cell–Related Injury. HemaSphere, 7(3), e848.

Publication 2023

B220 PE-Cy7 CD3 FITC CD4 PE CD8 PerCP-Cy5.5

Antibodies Cy5 5 Fitc Flow cytometric Kidney

Corresponding Organization : University of Verona

Other organizations : University of Chieti-Pescara, University of Turin, Établissement Français du Sang, Délégation Paris 7, Université Paris Cité, Hôpital Saint-Louis, Assistance Publique – Hôpitaux de Paris, Inserm, Ceinge Biotecnologie Avanzate (Italy), University of Naples Federico II

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Protocol cited in 2 other protocols

Variable analysis

independent variables

Right kidney harvested

dependent variables

Flow cytometric analysis

control variables

Antibodies used: anti CD45-V450, Ly6G APC-Cy7, B220 PE-Cy7, CD3 FITC, CD4 PE, CD8 PerCP-Cy5.5 (ThermoFisher Scientific, Waltham)

controls

No specific positive or negative controls were mentioned in the provided information.

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