Immunohistochemical Analysis of Lungs

Lungs were fixed in formalin for histologic analysis or used for bronchial alveolar lavage (BAL) collection (18 (link)). Sections were processed for H&E staining or immunohistochemistry staining with antibodies to CPT1 (8F6AE9 Abcam; 1:100) or HADHA (ab54477 Abcam; 1:500). Some sections were also stained with antibodies to CPT1 or HADHA together with F4/80 (SP115 Abcam; 1:100), Ly-6G (RB6–8C5 ebioscience; 1:500), Siglec-F (E50–2440 BD Biosciences; 1:500), or CD45R (RA3–6B2; BD Biosciences; 1:500). Protocols were described previously (7 (link)). BAL fluids were subjected to cytospin and stained with Diff-Quik (IMEB).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Al-Khami A.A., Ghonim M.A., Valle L.D., Ibba S.V., Zheng L., Pyakurel K., Okpechi S.C., Garay J., Wyczechowska D., Sanchez-Pino M.D., Rodriguez P.C., Boulares A.H, & Ochoa A.C. (2017). Fueling the Mechanisms of Asthma: Increased Fatty Acid Oxidation in Inflammatory Immune Cells May Represent a Novel Therapeutic Target. Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 47(9), 1170-1184.

Publication 2017

ab54477 Siglec-F E50–2440 CD45R RA3–6B2

Antibodies Bronchial alveolar lavage fluids Formalin Lungs Siglec

Corresponding Organization : Augusta University Health

Top 5 similar protocols

Variable analysis

independent variables

Fixation of lungs in formalin for histologic analysis
Use of lungs for bronchial alveolar lavage (BAL) collection

dependent variables

H&E staining of lung sections
Immunohistochemistry staining of lung sections with antibodies to CPT1 and HADHA
Immunohistochemistry staining of lung sections with antibodies to CPT1 or HADHA together with F4/80, Ly-6G, Siglec-F, or CD45R
Cytospin and Diff-Quik staining of BAL fluids

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!